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This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.。关于这个话题，搜狗输入法下载提供了深入分析
第七十五条 有下列行为之一的，处警告或者五百元以下罚款；情节较重的，处五日以上十日以下拘留，并处五百元以上一千元以下罚款：，更多细节参见im钱包官方下载